EVALUATION OF RECOMBINANT LEPTOSPIRA INTERROGANS SEROVAR CANICOLA OUTER MEMBRANE PROTEINS AS DIAGNOSTIC ANTIGEN
نویسندگان
چکیده
منابع مشابه
Evaluation of recombinant Leptospira interrogans serovar canicola outer membrane proteins as diagnostic antigen.
Leptospirosis of animals and man is an important rereported in recent years are LipL32, LipL41 and OmpL1; they emerging infectious disease worldwide. In domestic animals are expressed in-vivo during infection. LipL32 and LipL41 it is an important cause of abortion, stillbirth, infertility, are important and surface-exposed leptospiral outer membrane decreased milk production and death. In dairy...
متن کاملImmunoreactive outer membrane proteins of Leptospira interrogans serovar Canicola strain Hond Utrecht IV.
BACKGROUND & OBJECTIVES Leptospirosis is a severe and complex zoonotic disease prevalent in many countries including India. Current leptospiral research is focussed on the identification of the outer membrane proteins (OMPs) of the organism that could be used in developing diagnostic assays for leptospirosis. METHODS The Leptospira interrogans serovar Canicola was grown in EMJH medium and the...
متن کاملExpression and characterization of recombinant leptospiral outer membrane protein LipL32 from Leptospira interrogans serovar autumnalis.
Leptospira interrogans serovar autumnalis, a causative agent of leptospirosis in Thailand, was isolated from a patient for DNA extraction and amplification of LipL32 gene by polymerase chain reaction (PCR). The 782 bp PCR product was obtained, which was inserted into pAE plasmid with polyhistidine (His6 tag) to construct pAE-LipL32. This recombinant plasmid was transfected into E. coli BL21 (DE...
متن کاملamplification and cloning of a gene encoding a 41 kda outer membrane protein (lipl41) of leptospira interrogans serovar canicola
background: leptospirosis has been recognized as an important reemerging infectious disease caused by pathogenic leptospira spp. a major challenge of this disease is the application of a basic research to improve diagnostic method. outer membrane proteins of leptospira are potential candidates that could be useful in diagnosis. among them the lipl41 is an immunogenic protein which is present on...
متن کاملCharacterisation of the Proteome of Leptospira interrogans Serovar Canicola as a Resource for the Identification of Common Serovar Immunogenic Proteins
Over 230 serovars of Leptospira interrogans have been identified; however few have been completely characterised. The aim of this study was to characterise the proteome of serovar Canicola and to compare this against the serovars of Copenhageni and Pomona. 2D-LC/MS analysis identified 1653 Leptospira proteins in serovar Canicola; 60 of these proteins were common to Copenhageni and Pomona, 16 of...
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ژورنال
عنوان ژورنال: Indian Journal of Medical Microbiology
سال: 2006
ISSN: 0255-0857
DOI: 10.1016/s0255-0857(21)02319-7